Uncategorized

Synthetic biologics present a path to target bHLH transcription elements

The skill to target nearly all of human transcription elements remains an as yet unachieved aim in chemical biology and medication. We developed a modular, synthetic transcriptional repressor platform that recapitulates the DNA-binding properties of native frequent helix-loop-helix (bHLH) domains and could perhaps block the DNA-binding functions of a indubitably famous oncogenic transcription factor target, MYC.

That is a preview of subscription train, access by device of your institution

Gain entry to choices

Subscribe to Nature+

Gain instantaneous on-line access to Nature and 55 other Nature journal

Subscribe to Journal

Gain fat journal access for 1 twelve months

$99.00

completely $8.25 per grief

All costs are NET costs.

VAT shall be added later within the checkout.

Tax calculation shall be finalised real by device of checkout.

Retract article

Gain time restricted or fat article access on ReadCube.

$32.00

All costs are NET costs.

Additional access choices:

Log in

Learn about institutional subscriptions

Fig. 1: Modular arrangement and synthesis of man-made transcriptional repressors.

ReferencesLambert, S. A. et al. The human transcription elements. Cell 172, 650–665 (2018). This overview gifts a world description of transcription elements within the human proteome.

Article 
CAS 
PubMed 

Google Student 

Madden, S. K., de Araujo, A. D., Gerhardt, M., Fairlie, D. P. & Mason, J. M. Taking the Myc out of cancer: in the direction of therapeutic programs to straight away inhibit c-Myc. Mol. Most cancers 20, 3 (2021). This overview describes MYC in cancer and approaches to block verbalize.

Article 
PubMed 
PubMed Central 

Google Student 

Canne, L. E., Ferre-D’Amare, A. R., Burley, S. K. & Kent, S. B. H. Whole chemical synthesis of a optimistic transcription factor-connected protein: cMyc-Max. J. Am. Chem. Soc. 117, 2998–3007 (1995). This paper experiences the first chemical synthesis of a bHLH heterodimer.

Article 
CAS 

Google Student 

Talanian, R. V., McKnight, C. J. & Kim, P. S. Sequence-particular DNA binding by a short peptide dimer. Science 249, 769–771 (1990). This paper experiences binding to DNA by a dimerized leucine zipper peptide.

Article 
CAS 
PubMed 

Google Student 

Pajic, A. et al. Cell cycle activation by c-myc in a Burkitt lymphoma model cell line. Int. J. Most cancers 87, 787–793 (2000). This paper experiences the modification of a B cell line for preserve watch over of MYC expression.

Article 
CAS 
PubMed 

Google Student 

Download references

Additional informationPublisher’s bid Springer Nature remains impartial with regards to jurisdictional claims in printed maps and institutional affiliations.

That is a summary of: Speltz, T. E. et al. Focusing on MYC with modular synthetic transcriptional repressors derived from bHLH DNA-binding domains. Nat. Biotechnol. https://doi.org/10.1038/s41587-022-01504-x (2022).

About this article

Cite this article Synthetic biologics present a path to target bHLH transcription elements.
Nat Biotechnol (2022). https://doi.org/10.1038/s41587-022-01509-6

Download quotation

Published: 03 November 2022

DOI: https://doi.org/10.1038/s41587-022-01509-6

Related Articles

Leave a Reply

Your email address will not be published. Required fields are marked *

Back to top button